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https://doi.org/10.5194/os-2020-2
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/os-2020-2
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Submitted as: research article 17 Jan 2020

Submitted as: research article | 17 Jan 2020

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This preprint is currently under review for the journal OS.

A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined to HPAEC-PAD

Sebastian Zeppenfeld1, Manuela van Pinxteren1, Anja Engel2, and Hartmut Herrmann1 Sebastian Zeppenfeld et al.
  • 1Atmospheric Chemistry Department(ACD), Leibniz-Institute for Tropospheric Research (TROPOS), Leipzig, Germany
  • 2GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany

Abstract. An optimized method is presented to determine free (DFCHO) and combined monosaccharides (CCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination between desalination with electro-dialysis and high performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95–98 % recovery rates. Free amino sugars and uronic acids were strongly depleted during electro-dialysis at pH = 8, but an adjustment of the pH could result in higher recoveries (58–59 % for amino sugars at pH = 11; 45–49 % for uronic acids at pH = 1.5). The applicability of this method for the analysis of CCHO was evaluated with standard solution and real seawater samples compared with another established desalination method using membrane dialysis. DFCHO in real field samples from different regions on earth ranged between 11–118 nM and CCHO between 260–1410 nM. This novel method potentially contributes to a better understanding of biogeochemical processes in the oceans and sea-air transfer processes of organic matter into the atmosphere during further research studies.

Sebastian Zeppenfeld et al.

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Free neutral monosaccharides and combined monosaccharides in seawater and related saline matrices S. Zeppenfeld, M. van Pinxteren, and H. Herrmann https://doi.pangaea.de/10.1594/PANGAEA.910575

Sebastian Zeppenfeld et al.

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Short summary
An analytical method combining electro-dialysis with high performance anionic exchange chromatography coupled to pulsed amperometric detection was developed and optimized for analysing free and combined monosaccharides in seawater and other saline environmental samples.
An analytical method combining electro-dialysis with high performance anionic exchange...
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